Submission procedure
Before submitting samples, you must contact LMSR personnel to set up appropriate logistics. Some lipids are unstable and require immediate analysis. All samples must be accompanied by a sample submission form via SmartSheets. For more information on sample shipping protocols, please refer to this link.
Process
The LMSR works with a variety of samples including serum, plasma, tissues, cells and cell media (dye-free preferred). Samples are extracted using a modified Bligh-Dyer followed by the requested quantitative analysis. All data will be delivered to the submitting individual and laboratory PI as an easy-to-read Excel file.
Targeted lipids:
- Uses internal standards and methods to yield highly sensitive, accurate data on species of interest within lipid classes
- Allows for quantification utilizing three separate identifiers for each analyte
- Internal standards
- Structure-specific fragmentation
- UHPLC retention times
- Many species within a lipid class can be quantitated in a single run while separating stereoisomers and potential isobaric contaminants
Targeted metabolites:
- Targeted – interested metabolites impacted by stressors
- Targeted biochemical pathway information
- Sample measurement (FDA standards)
- Concentration, result is normalized by total protein or tissue weight, etc.
Rates
Download our current rates
Note: External academic pricing is equal to VCU Pricing + fringe (55%)
Services
- Targeted Analysis
- Lipid Species Classes
- Cardiolipin Analysis
- Eicosanoid Analysis
- Ganglioside GM3 Analysis
- LPA Analysis
- Phospholipid Analysis
- Sphingolipids
- Sphingosine, Sphinganine, Sphingosine-1-phosphate, Sphinganine-1-phosphate,
- Ceramide
- Monohexosylceramide
- Sphingomyelin
- Dihydrosphingolipids
- The Core is able to accommodate analysis of other sphingoid basis and N-acyl fatty acids upon request
- Sphingolipid Kinase Assay
- Steroid Hormone Analysis
- Water-Soluble Specific Classes
- Metabolites
- Lactate, pyruvate, glucose
- Amino Acids
- Glutathione
- Nucleotides
- MitoQ
- Vitamin D Analysis
- Metabolic pathway:
TCA cycle
- Glycolysis/gluconeogenesis
- Pentose Phosphate Pathway
- One Carbon Pathway
- Custom Pathway Metabolites
- The LMSR is also available for consultation for development of targeted LC-MS/MS methodologies for water-soluble metabolites/pathways not currently offered.
- Isotopic labeling/ metabolite tracing
- Untargeted Analysis
- Full Spectrum Lipidomics Analysis
- Full Spectrum Metabolomics Analysis
- Additional Services
- Method Development
- If analysis is needed for compounds not listed above, please contact us, as we may be able to develop an appropriate method to suit your needs.
- Instrument Time
Certified users are granted access to scheduling time to run and analyze their own samples on a per hour basis.
- Total Lipid Phosphate Assay
- Agilent Seahorse
- Technician Time
- Priced per hour work by core staff
- Consultation
- Training Session
- Certified User
- Data Analysis
Massey member subsidy benefit
Massey subsidizes 25 percent of the cost of members’ core laboratory usage for the following Massey-supported, fee-for-service shared resources:
- Flow Cytometry Shared Resource
- Lipidomics & Metabolomics Shared Resource
- Microscopy Shared Resource
- Tissue and Data Acquisition and Analysis Core
- Transgenic/Knockout Mouse Shared Resource
- Cancer Mouse Models Core
- Proteomics Shared Resource
To receive this member benefit, please initiate core lab services using a VCU index code associated with your name. When you are billed for core laboratory usage, the Rams FORCE system will automatically split the invoice between your index code and a Massey account established for this purpose. You will be charged 75 percent of the published rate and Massey will provide a 25 percent co-pay to cover the balance. Please direct any questions or concerns about the member subsidy benefit to Paul Fawcett, Ph.D. at paul.fawcett@vcuhealth.org or (804) 827-0975.
*Reduced rates and pricing are also available for LMSR certified users at a No-technician rate.
